Journal: Bioconjugate chemistry

Article Title: Simple Method To Prepare Oligonucleotide-Conjugated Antibodies and Its Application in Multiplex Protein Detection in Single Cells.

doi: 10.1021/acs.bioconjchem.5b00613

Figure Lengend Snippet: Fig. 6. Detection of proteins in cell lysates. A panel of 24-plex antibody binders was used to detect their respective target proteins in MCF7, K562, A549, and A431 cell lysates. The cell lysates were diluted to different concentrations and reacted with a mixture of antibody binders. Only selected examples of protein targets CSTB (A), CASP3 (B), Ki-67(C), and GATA3 (D) are shown. Ct values were shown as the means ± SD. Ct: cycle threshold.

Article Snippet: MCF7, K562, A549, and A431 cell lysates were obtained from Novus (Littleton, CO).

Techniques:

Journal: BMC cancer

Article Title: Identification of the angiogenic gene signature induced by EGF and hypoxia in colorectal cancer.

doi: 10.1186/1471-2407-13-518

Figure Lengend Snippet: Figure 5 EGF receptor is autophosphorylated in Caco-2 and activates downstream signalling pathways. Caco-2 cells were stimulated with 20 ng/mL EGF for the time periods indicated. Western blotting for (a) phosphorylated EGFR or total EGFR and (b) antibodies recognising signal- ling enzymes is shown. Cell lysate of EGF-treated A431 cells was used as positive control. α-tubulin is shown as a loading control. Densitometry was performed using Phoretix 1D analysis software against α-tubulin (for ERK, data for p42 and p44 are shown).

Article Snippet: Whole cell lysate of EGF-treated A431 epithelial carcinoma cells used as positive control was from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Western Blot, Positive Control, Control, Software

Journal: bioRxiv

Article Title: Local albumin excess exacerbates Candida albicans-induced inflammasome activation linked with hyperinflammation during vulvovaginal candidiasis

doi: 10.64898/2026.01.22.700771

Figure Lengend Snippet: (A) Cytokine release by A-431 cells ( n = 5) in response to C. albicans infection in the presence or absence of albumin at 24 hpi. (B) Cytokine release by SW954 cells ( n = 4) in response to C. albicans infection in the presence or absence of albumin at 24hpi. (C) Cytokine release by human monocyte-derived macrophages ( n = 8) in response to C. albicans in the presence or absence of albumin. ( D ) IL-1β release by human monocyte-derived macrophages ( n = 8) in response to C. albicans in the presence or absence of albumin and/or anakinra ( n = 7). (E) IL-1β levels in culture supernatants of neutrophils ( n = 5) in response to C. albicans in the presence or absence of albumin and/or supplemented IL-1β. Data are from at least three independently conducted experiments. Bars represent the mean + SEM with dots as individual biological replicates. Statistical analysis was done using a two-way ANOVA. * = P ≤ 0.05, ** = P ≤ 0.01, *** = P ≤ 0.001, **** = P ≤ 0.0001.

Article Snippet: To mimic vulvovaginal mucosal infection, A-431 cells (VECs, Deutsche Sammlung von Mikroorganismen und Zellkulturen DSMZ no. ACC 91) were used.

Techniques: Infection, Derivative Assay

Journal:

Article Title: Enteropathogenic Escherichia coli -Induced Epidermal Growth Factor Receptor Activation Contributes to Physiological Alterations in Intestinal Epithelial Cells ▿

doi: 10.1128/IAI.01690-06

Figure Lengend Snippet: EPEC-induced EGFR activation is delayed in polarized epithelial cells. (A) Polarized C2BBE cells grown on permeable supports were infected with WT EPEC, and the extracts isolated at specific time points were immunoblotted against EGFR pY1068. EGF-treated A431 cell extract was included as a positive control to confirm the identity of the band. (B) Data from three similar experiments were evaluated by densitometry (P < 0.005 by analysis of variance for 240 min).

Article Snippet: Control EGF-treated A431 cell extracts and antibody to total EGFR were obtained from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques: Activation Assay, Infection, Isolation, Positive Control